Thymosin Beta 4 (TB-500) Peptide

Thymosin Beta 4 (TB-500) – Synthetic Peptide for Tissue Repair and Regeneration Research

Basic Information

• Product Name: Thymosin Beta 4 (TB-500)

• CAS Number: 77591-33-4

• Molecular Formula: C₂₃₈H₃₅₀N₆₀O₇₈S

• Molecular Weight: 4963.56 g/mol

• Purity: ≥98% (HPLC)

• Appearance: White lyophilized powder

• Packaging: Available in vials (2 mg, 5 mg, 10 mg)

Overview
TB-500, the synthetic version of Thymosin Beta 4 (Tβ4), is a 43‑amino acid peptide that plays a critical role in tissue repair, cell migration, and angiogenesis. Thymosin Beta 4 is a naturally occurring peptide found in all cells, with particularly high concentrations in blood platelets, macrophages, and wound tissue. It is the primary intracellular G‑actin sequestering peptide, which controls the availability of actin for polymerization.

Mechanism of Action
Thymosin Beta 4 binds to G‑actin and promotes cell migration, proliferation, and differentiation. It also acts as a potent anti‑inflammatory agent and angiogenic regulator by:

• Stimulating vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs) to promote new blood vessel formation.

• Preventing polymorphonuclear leukocyte infiltration, reducing inflammation.

• Protecting hair cells and promoting corneal wound healing.

• Modulating cytokines to reduce inflammation in autoimmune conditions.

Key Research Applications

• Wound healing: Accelerates wound closure, promotes angiogenesis, and reduces inflammation in skin and corneal injuries.

• Muscle regeneration: Enhances satellite cell proliferation and myofiber formation, studied in models of muscle injury and ischemia.

• Cardiovascular research: Protects heart tissue from ischemic injury and improves cardiac function after myocardial infarction by promoting revascularization and reducing fibrosis.

• Neuroprotection: Explored for neurogenesis and protection in models of stroke and spinal cord injury.

• Anti‑inflammatory and immune studies: Reduces cytokines in conditions like colitis, pancreatitis, and osteoarthritis.

• Tissue engineering: Used as a bioactive component in hydrogels, scaffolds, and drug delivery systems.

Dosage and Administration
For research use only. Typical research dosages range from 5–20 µg/kg per day (rodent models) or 2–10 mg per day (human cell studies). Reconstitute in sterile water, bacteriostatic water, or 0.9% sodium chloride (500–1000 µL per 2–5 mg vial). Administer via subcutaneous, intramuscular, or intraperitoneal injection. Dosage should be determined based on specific study protocols.

Handling and Storage

• Store lyophilized powder at –20°C (up to 3 years) or –80°C (up to 5 years) in tightly sealed containers, protected from light and moisture.

• After reconstitution, store at –20°C for short‑term use (up to 2 weeks) or –80°C for long‑term storage; avoid repeated freeze‑thaw cycles.

• Use appropriate personal protective equipment (gloves, lab coat, safety goggles) when handling.

Quality Assurance
Each batch is tested for purity (by HPLC ≥98%), identity (by mass spectrometry), and sequence confirmation to ensure consistent quality.