PEG-MGF (PEGylated Mechano Growth Factor)

PEG-MGF (Pegylated Mechano Growth Factor) – Research-Grade Peptide for Muscle Repair and Regeneration Studies

Basic Information

• Product Name: PEG-MGF (Pegylated Mechano Growth Factor)

• Synonyms: Pegylated MGF, PEG IGF-1 Ec, Mechano Growth Factor, MGF, IGF-1EC

• CAS Number: 108174-48-7 (commonly referenced); also cited as 12629-01-5, 1015036-03-4, and 12020-86-9 (varies by manufacturer)

• Molecular Formula: C₁₂₁H₂₀₀N₄₂O₃₉ (common reference); other sources cite C₁₆₀H₂₆₉N₄₅₀₄₉ or C₁₅₉H₂₅₈N₄₆O₄₅

• Molecular Weight: ~2948.15 g/mol (peptide core); with PEG chain varies, total ~3824.5 g/mol

• Appearance: White to off-white lyophilized powder

• Purity: ≥98% (HPLC)

• Sequence (one-letter code): PEG-Suc-YQPPSTNKNTKSQ(d)R(d)RKGSTFEEHK-NH2

• Storage: Store lyophilized at –20°C, dry and protected from light

• Shelf Life: 24 months when stored properly

Overview
PEG-MGF (Pegylated Mechano Growth Factor) is a synthetic peptide derivative of Mechano Growth Factor (MGF), a splice variant of the insulin-like growth factor 1 (IGF-1) gene. MGF is produced naturally by muscle cells in response to mechanical stimulation (such as resistance training) or following muscle injury, serving as a crucial local repair and growth signal.

The native MGF peptide has an extremely short half‑life of only 5–7 minutes. To overcome this limitation, PEG-MGF is created by covalently attaching polyethylene glycol (PEG) chains to the peptide. This PEGylation process provides a protective coating that significantly prolongs the peptide's stability and half‑life in experimental systems, allowing for sustained biological activity.

Mechanism of Action
PEG-MGF acts by binding to IGF-1 receptors on muscle satellite cells, stimulating their activation and entry into the cell cycle. It promotes myoblast proliferation and differentiation, allowing muscle fibers to fuse and mature. Research has demonstrated that PEG-MGF helps increase the muscle stem cell count, enabling more cells to fuse and become part of adult muscle tissue. The PEGylation advantage maintains bioactivity for extended periods in vivo.

Key Benefits

• Stimulates satellite cell activation and myoblast proliferation

• Promotes muscle hypertrophy and protein synthesis

• Supports tissue repair and regeneration

• Extended half‑life compared to native MGF

• Enhanced stability and resistance to proteolytic degradation

• Suitable for in vitro and preclinical research applications

Applications

• Muscle growth and repair research: Investigates skeletal muscle regeneration, hypertrophy, and adaptation to mechanical stimuli

• Tissue engineering: Incorporated into biomaterial scaffolds to study cellular proliferation, migration, and matrix remodeling

• Regenerative medicine: Explored for sarcopenia, muscle wasting, and injury recovery

• Neurological research: Studied for neuroprotective and cardioprotective properties

• Signal pathway analysis: Used to dissect IGF-1 isoform signaling networks and receptor-mediated activation

• Pharmacokinetic modeling: Serves as a model system for studying PEGylation effects on peptide stability and biodistribution

Recommended Use
For research use only. Not for human consumption, therapeutic, or diagnostic use. Typical in vitro concentrations and dosages should be determined based on specific study protocols.

Handling and Storage

• Store lyophilized powder at –20°C in tightly sealed containers, protected from light and moisture

• After reconstitution, store at –80°C

• Avoid repeated freeze‑thaw cycles

• Use appropriate personal protective equipment (gloves, lab coat, safety goggles) when handling

• Reconstitute in sterile water or suitable buffer as required

Packaging
Available in 2 mg, 5 mg, and 10 mg vials. Custom packaging upon request.

Quality Assurance
Each batch is tested for purity (by HPLC ≥98%), identity (by mass spectrometry), and sequence confirmation to ensure consistent quality.